An object of the present invention is to provide a nucleic acid delivery carrier, a nucleic acid delivery kit, and a nucleic acid delivery method that have an excellent effect of suppressing cell death after delivery of nucleic acid and that are excellent in the efficiency of delivery of nucleic acid into cells. It is. The carrier for nucleic acid delivery of the present invention is a carrier for nucleic acid delivery into cells composed of liposomes having membrane fusion performance. The liposome contains a cationic lipid as a membrane constituent, and the surface of the liposome is temperature-responsive. It has been modified with a polymer. The carrier for nucleic acid delivery of the present invention is suitably used for delivery of nucleic acid into cervical cancer cells. The nucleic acid delivery kit of the present invention is a kit for delivering a nucleic acid into a cell, comprising the delivery carrier described above. The nucleic acid delivery method of the present invention comprises the step of bringing the above-mentioned nucleic acid delivery carrier and nucleic acid complex into contact with an isolated cell, or the above nucleic acid delivery carrier and nucleic acid complex from a human. A method for delivering a nucleic acid into a cell, comprising a step of administering to a non-animal.本発明の課題は、核酸送達後の細胞の死滅の抑制効果に優れ、かつ、核酸の細胞内への送達効率に優れた核酸送達用キャリア、核酸送達用キット、及び核酸送達方法を提供することである。 本発明の核酸送達用キャリアは、膜融合性能を有するリポソームからなる、細胞内への核酸送達用キャリアであって、リポソームは、膜構成成分としてカチオン性脂質を含み、リポソームの表面が温度応答性ポリマーで修飾されたものである。本発明の核酸送達用キャリアは、子宮頸癌細胞内への核酸の送達に好適に用いられる。本発明の核酸送達用キットは、上記の送達用キャリアを含む、細胞内への核酸送達用キットである。本発明の核酸送達方法は、上記の核酸送達用キャリアと核酸との複合体と、単離された細胞とを接触させる工程、又は、上記の核酸送達用キャリアと核酸との複合体をヒトを除く動物に投与する工程を有する、細胞内への核酸送達方法である。
