According to the present invention, a low-temperature sterilization method can comprise: (S100) a first vacuum step of locating an object (14), to be sterilized, sealed in a sealing member (13), in a sterilization space (11) within a chamber (10), and then reducing the pressure of the sterilization space (11) to a pressure lower than atmospheric pressure (S200) a plasma treatment step of injecting plasma-treated external air into the sterilization space (11) (S300) a pretreatment step of circulating the air of the inner space (15) of the sealing member and the air of the sterilization space (11) by changing the pressure of the inside of the sterilization space (11) through a change in volume of a variable sterilization space (12) (S400) a second vacuum step of reducing the pressure inside the sterilization space (11) to a pressure lower than that of the pretreatment step (S300) (S500) a sterilizing agent injection step of injecting a sterilizing agent into the sterilization space (11) (S600) a sterilization step of injecting a sterilizing agent into the sealing member (13) by changing the pressure of the sterilization space (11) through a change in volume of the variable sterilization space (12) and (S700) an external air injection step of injecting external air into the sterilization space (11). According to the present invention, the volume of the chamber providing the sterilization space is continuously changed, thereby allowing the pressure therein to be adjusted, and thus a sterilization effect can be improved through the effective penetration and supply of a sterilizing agent.La présente invention concerne un procédé de stérilisation à basse température, qui peut comprendre les étapes suivantes : (S100) une première étape dapplication de vide consistant à placer un objet (14) à stériliser, isolé dans un élément détanchéité (13), dans un espace de stérilisation (11) à lintérieur dune chambre (10), puis à faire baisser la pression de lespace de stérilisation (11
