THE GENERAL HOSPITAL CORPORATION;PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人:
LESSER, CAMMIE,WAGERS, AMY JO,REEVES, ANALISE Z.
申请号：
EP14760175
公开号：
EP2964240A4
申请日:
2014.03.06
申请国别(地区):
EP
年份:
2016
代理人:
摘要：
The methods and compositions described herein relate to the delivery of polypeptides to a target cell, e.g. by utilizing engineered non-pathogenic bacteria comprising a type three secretion system (T3SS) and T3SS-compatible substrates. In one aspect, described herein are non-pathogenic microbial cells that have been engineered to express both a functional type three secretion system (T3SS) and at least one polypeptide that is compatible with the T3SS. Due to the wide variety of polypeptides that can be delivered to a target eukaryotic cell using the compositions and systems described herein, a commensurately wide variety of applications is contemplated, e.g. therapeutics and reprogramming.
