The State of Israel; Ministry of Agriculture & Rural Development; Argricultural Research Organiza
发明人:
Maxim ITKIN,Rachel DAVIDOVICH-RIKANATI,Shahar COHEN,Vitaly PORTNOY,Adi DORON-FAIGENBOIM,Marina PETREIKOV,Shmuel SHEN,Yaakov TADMOR,Yosef BURGER,Efraim LEWINSOHN,Nurit KATZIR,Arthur A. SCHAFFER,Elad OR
申请号：
US15510708
公开号：
US20170283844A1
申请日:
2015.09.10
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT). Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.
