The present invention provides a technique for conveniently and efficiently regenerating elastic fiber retaining a normal structure, and a technique enabling preparation of an artificial tissue comprising elastic fiber (for example, artificial skin, artificial blood vessels) that can be transplanted to humans. Specifically, the invention provides a method of producing elastic fiber, comprising culturing (for example, culturing in serum-free medium) cells having the capability of regenerating elastic fiber in the presence of DANCE and/or fibulin-4； artificial elastic fiber comprising DANCE and/or fibulin-4； an elastic fiber regenerating agent； a serum-free medium comprising DANCE and/or fibulin-4； and cells having the capability of regenerating elastic fiber, transfected with at least one of a DANCE expression vector, a fibulin-4 expression vector and a DANCE inducing factor expression vector, and the like.
