The present invention relates to a humidifier sterilization composition using natural ingredients and a production method thereof, and more specifically, to a humidifier sterilization composition which is harmless to the human body by using natural ingredients, exhibits an excellent sterilizing effect, and is transparent to prevent the coloring when being applied to the humidifier; and to a production method of the humidifier sterilization composition. The humidifier sterilization composition is prepared by using at least one medicinal herb selected from the group consisting of Illicium verum, fennel seeds, Scutellaria baicalensis, and a combination thereof.본 발명은 천연 가습기 살균 조성물 및 그의 제조방법에 관한 것으로서, 보다 상세하게는, 천연 원료를 사용하여 인체 무해하고, 살균효과가 우수하며, 가습기 적용시 착색의 우려가 없도록 무색 투명한 천연 가습기 살균 조성물 및 그의 제조방법에 관한 것이다.