Gentschev, Ivaylo,Goebel, Werner,Fensterle, Joachim,Rapp, Ulf R.
申请号：
AU2007321266
公开号：
AU2007321266B2
申请日:
2007.11.13
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
The invention relates to a microorganism as a carrier of nucleotide sequences codingfor antigens and protein toxins comprising the following components: (I) atleast one nucleotide sequence coding for at least one complete or partial antigenof at least one wild-type or mutated protein； and (II) at least one nucleotide sequencecoding for at least one protein toxin and/or at least one protein toxin subunit；and (III) a) at least one nucleotide sequence coding for at least one transportsystem which enables the expression of the expression products of component(I) and component (II) on the outer surface of the microorganism and/or enablesthe secretion of the expression products of component (I) and component (II)；and/or coding for at least one signal sequence which enables the secretion ofthe expression products of component (I) and component (II)； and/or (III) b)optionally, at least one nucleotide sequence coding for at least one protein forlysing the microorganism in the cytosol of mammalian cells and for intracellularly releasingplasmids or expression vectors, which are contained in the lysed microorganism； and(IV) at least one nucleotide sequence for at least one activation sequence forthe expression of one or more of components (I) to (III), wherein said activation sequencecan be activated in the microorganism and/or is tissue cell-specific, tumorcell-specific, macrophage-specific, dendrite-specific, lymphocyte-specific, function-specificor non-cell-specific"； wherein any of components (I) to (IV) can be presenteither once or several times and either identical or different. Also disclosedare a process of manufacturing thereof, corresponding plasmids or expressionvectors and uses of the microorganism as a medicament.
