The present invention is to provide a two-stage sterilization process for treating heat-resistant spores in a great burdock extract drink, which comprises the steps of sealing dried great burdock and water in a double-kettle pressure cooker heating liquid mixture of great burdock and water to a first temperature range lower than or equal to 100° C. for at least a first duration during a first-stage sterilization opening lid of the cooker and filtering out great burdock residue so that all that remains in the cooker is great burdock extract resealing the cooker and heating the great burdock extract to a second temperature range higher than or equal to 120° C. for at least a second duration during a second-stage sterilization, and the pressure in the cooker during the second-stage sterilization is higher than that during the first-stage sterilization so as to obtain the extract drink free of common bacteria and heat-resistant spores.本發明係一種處理牛蒡萃取物飲料中耐熱性孢子之兩階段式殺菌製程,該殺菌製程係先將乾牛蒡與水密封至一二重釜壓力鍋內,進行第一階段之低溫低壓殺菌作業,俟牛蒡與水之混合液被加熱至一第一溫度範圍後,持續第一期間以上即停止加熱;嗣,開啟鍋蓋,濾除牛蒡渣,使鍋內僅剩萃取液後重新密封;執行第二階段之高溫高壓殺菌作業,俟萃取液被加熱至第二溫度範圍後,且持續第二期間以上即停止加熱;最後,俟萃取液冷卻至常溫後,即能獲得完全無細菌及耐熱性孢子之牛蒡萃取物飲料,且能透過不同溫度範圍之二階段殺菌作業,保留絕佳之原始風味。31~35‧‧‧步驟