The invention provides an edible composition as well as a preparation method and application thereof. The edible composition comprises functional oligosaccharides and probiotics, wherein the functional oligosaccharides and the probiotics have synergistic effects and can conduce to obviously increasing the number of intestinal probiotics of animals and inhibiting increase of harmful bacteria and harmful substances, thus effectively regulating intestinal floras of animals and then enhancing the immunities based on the effects of the intestinal floras and preventing or treating the diseases, such as obesity, diabetes mellitus, hypertension, hyperlipidemia or tumors.