The invention provides an edible composition as well as a preparation method and application thereof. The edible composition comprises inulin and clostridium butyricum in a weight ratio of (1.2-2) to 1. The intestinal floras of animals can be effectively regulated by feeding the animals with the edible composition, thus enhancing the immunities of the animals and preventing or treating the diseases, such as obesity, diabetes mellitus, hypertension, hyperlipidemia or tumors.