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METHOD FOR PRODUCING ERYTHROID CELLS, METHOD FOR PRODUCING BLOOD PRODUCT, CELL POPULATION, COMPOSITION OF FROZEN CELLS AND KIT FOR PRODUCING HEMATOPOLESE OR CELL PROGENITOR CELLS
专利权人:
THE UNIVERSITY OF TOKYO
发明人:
HIROMITSU NAKAUCHI,KOJI ETO,NAOYA TAKAYAMA,SOU NAKAMURA
申请号:
BR122013008030
公开号:
BR122013008030A2
申请日:
2010.09.15
申请国别(地区):
BR
年份:
2019
代理人:
摘要:
method for producing erythroid cells, method for producing a blood product, cell population, frozen cell composition and kit for producing hematopoietic progenitor cells or erythroid cells. Method for producing erythroid cells characterized in that it comprises: forcibly expressing an oncogene and or a hoxa2 gene or a bclxl gene in hematopoietic progenitor cells, cd34 positive cells, erythroid progenitor cells or pre-anucleated glycophorin pre-anucleated cells and growth of cells; and differentiate cells grown into erythroid cells. Method for producing erythroid cells according to Claim 1, characterized in that the differentiation step comprises: cultivating the grown cells by suppressing the expression of the oncogene and the hoxa2 gene or the bclxl gene; or culturing cells grown after the oncogene and the hoxa2 gene or bclxl gene have been removed from the cells. The method for producing erythroid cells according to claim 1, further comprising cryopreserving cells grown after the growth step, wherein the differentiation step is conducted after the cells are thawed. Method for producing erythroid cells according to any one of Claims 1 to 3, characterized in that the oncogene is selected from the genes of the myc family. A method for producing a blood product comprising: producing erythroid cells by the method according to any one of claims 1 to 4; and mixing erythroid cells with other components of a blood product. A cell population characterized by the fact that it comprises hematopoietic progenitor cells, cd34 positive cells, erythroid progenitor cells, or α-positive pre-anucleated gycophorin cells into which an oncogene and a hoxa2 gene or bclxl gene are introduced. Cell population according to claim 6, characterized in that the oncogene is selected from genes of the myc family. A frozen cell composition comprising the cell population according to claim 6 or 7. Kit for producing hematopoietic progenitor cells or erythroid cells, comprising expression vectors for
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