CHEN, KUAN-CHOU,OUYANG, TIANMEI,OJA, STEPHEN,FELDMAN, BENJAMIN J.,CHO, HYUN,TRAN, LAM,ESHOO, MARK
申请号：
CA3094050
公开号：
CA3094050A1
申请日:
2019.02.12
申请国别(地区):
CA
年份:
2019
代理人:
摘要：
A lactate-responsive enzyme may form the basis for lactate detection and quantification using an electrochemical analyte sensor. Various features may be incorporated within an analyte sensor containing a lactate-responsive enzyme, particularly lactate oxidase, to improve sensitivity and response stability of the analyte sensor. Such analyte sensors may comprise: a working electrode having an active area disposed thereon, and a mass transport limiting membrane overcoating at least the active area upon the working electrode. The active area comprises at least a polymer, an albumin, and a lactate-responsive enzyme that is covalently bonded to the polymer. The mass transport limiting membrane may comprise at least a crosslinked polyvinylpyridine homopolymer or copolymer. The analyte sensors may determine a lactate concentration in a biological fluid, particularly in vivo, which may be correlated to various physiological conditions.
