Obshchestvo s ogranichennoj otvetstvennostyu "BioMIKHM"
发明人:
German Lyudmila Sergeevna,Герман Людмила Сергеевна,Senatorova Valentina Nikolaevna,Сенаторова Валентина Николаевна,Vakar Lyubov Lvovna,Вакар Любовь Львовна,Biryukov Valentin Vasilevich,Бирюков Валенти
申请号：
RU2015121481
公开号：
RU0002613493C2
申请日:
2015.06.05
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
FIELD: agriculture.SUBSTANCE: invention relates to agriculture, namely to fodder production, and can be used in production of fodder yeast from hydrolysates, obtained during processing of vegetable raw material. Method includes preliminary processing of grain starch-containing material, preparation of nutrient medium consisting of carbohydrate and salt parts, cultivation of yeast culture on prepared nutrient medium in fermenter in continuous mode, further concentration and drying of yeast suspension to obtain end product. Preliminary processing of grain starch-containing material involves its crushing by wet method, separation of grain shells, extraction of gluten, serial enzymatic hydrolysis of remaining starch part with alpha-amylase and glucoamylase to obtain product with glucose content no less than 25 %, enzymatic hydrolysis with cellulolytic enzyme preparations containing xylonase and cellulase, separation of grain shells after preliminary thermo-chemical treatment to obtain product with glucose content of no less than 12 %. Obtained glucose-containing parts of nutrient medium are combined with mineral part of nutrient medium and successive culturing of yeast Hansenula polymorpha genus VKPM Y-314 in fermenter is performed in continuous mode Hansenula jadinii VKPM Y-797, Hansenula petersonii VKPM Y-1012 with subsequent concentration of yeast biomass, which is dried and granulated to obtain end product. Wherein initial and running medium for cultivation of second and further cultures of yeast is nutrient medium filtrate from previous fermentation process. For yeast Hansenula polymorpha VKPM Y-314 nutrient medium with content of reducing substances no less than 20 % is supplied. Recovered gluten, after additional cleaning, is released in form of ready product – fodder gluten with protein content no less than 70 %. Invention implementation provides production of protein-vitamin additive with high content of proteins, vitamins of group B and indispensable amino aci
