This invention is the method of being isolated the island by density centrifugal separation, it adds the island, in the solution which includes the island which possesses density-gradient the island it is isolated by centrifugal separating the tank, the pressure which falls on the island is under 50Pa, the island is the method of being isolated from slope, improvement, includes the method of including the fact that continual density-gradient is produced in the tank which at least includes 100 millilitres making use of the crookedness tube which decreases the flow of the convection which destroys slope.< Selective figure >Figure 1本発明は、膵島を密度遠心分離により単離する方法であり、膵島を、密度勾配を有する膵島を含む溶液中に添加し、槽を遠心分離することによって膵島を単離し、膵島にかかる圧力が50Pa未満であり、膵島を勾配から単離する方法であって、改良が、勾配を破壊する対流の流れを低下させる屈曲チューブを用いて、少なくとも100ミリリットルを含む槽中に連続的な密度勾配を生み出すことを含む方法を含む。【選択図】図1