Mark S. Payne,Stephen K. Picataggio,Amy Kuang-Hsu,Ramesh Velayudhan Nair,Fernando Valle,Philippe Soucaille,Donald Eugene Trimbur
申请号：
US12940225
公开号：
US08168608B2
申请日:
2010.11.05
申请国别(地区):
US
年份:
2012
代理人:
摘要：
This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.
