Manganese-containing yeast extraction comprising an extraction step of suspending a yeast containing manganese in a solution containing at least one of carboxylic acid and carboxylate and separating a solid component and a liquid component of the obtained suspension. Product manufacturing method, yeast extract with high manganese content, food, lactic acid bacteria culture medium composition for food, food lactic acid bacteria culture medium, and food lactic acid bacteria culture method.マンガンを含有する酵母を、カルボン酸及びカルボン酸塩の少なくともいずれかを含む溶液に懸濁させ、得られた懸濁液の固体成分と液体成分とを分離する抽出工程を含むマンガン高含有酵母抽出物の製造方法、及びマンガン高含有酵母抽出物、並びに、食品、食品用乳酸菌培養培地用組成物、食品用乳酸菌培養培地、及び食品用乳酸菌の培養方法である。