The present invention describes derivative polysaccharide-protein conjugate, the composition comprising one or more kinds of such polysaccharide derivative-protein conjugates and using the method for their immunity inoculation patients. These polysaccharide derivative-protein conjugates are the purified capsular polysaccharides from Neisseria meningitidis (Neisseria meningitidis) serogroups A, C, W-135 and Y, wherein these purified capsular polysaccharides are by derivative, chemically sensitized and be selectively conjugated with carrier protein by way of covalent chemical bond, so as to form the polysaccharide-protein conjugate that can cause for the permanent immunity of a variety of N. meningitidis stains.本發明描述了衍生的多糖-蛋白質綴合物、包含一種或一種以上此類衍生多糖-蛋白質綴合物的組合物以及使用它們免疫接種病人的方法。這些衍生多糖-蛋白質綴合物是來自腦膜炎奈瑟氏球菌(Neisseria meningitidis)血清群A、C、W-135和Y的純化莢膜多 糖，其中這些純化莢膜多糖是經過衍生的、化學激活的和通過共價化學鍵的方式選擇性地與載體蛋白質綴合的，從而形成了能够引起針對多種腦膜炎奈瑟氏球菌菌株長久免疫性的多糖-蛋白質綴合物。
