CHINESE ACADEMY OF SCIENCES;INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY
发明人:
GAO, CAIXIA,WANG, YANPENG,ZHANG, YI,LIU, JINXING,ZHANG, KANG
申请号:
CA2973750
公开号:
CA2973750A1
申请日:
2016.01.19
申请国别(地区):
CA
年份:
2016
代理人:
摘要:
Provided is a method for conducting site-specific modification in a plant through gene transient expression, comprising the following steps: transiently expressing a sequence-specific nuclease specific to the target fragment in the cell or tissue of the plant of interest, wherein the sequence-specific nuclease is specific to the target site and the target site is cleaved by the nuclease, thereby the site-specific modification of the target site is achieved through DNA repairing of the plant.