Chinese Academy of Sciences;Institute of Genetics and Developmental Biology
发明人:
Gao, Caixia,Wang, Yanpeng,Zhang, Yi,Liu, Jinxing,Zhang, Kang
申请号:
AU2016208913
公开号:
AU2016208913A1
申请日:
2016.01.19
申请国别(地区):
AU
年份:
2017
代理人:
摘要:
Provided is a method for conducting site-specific modification in a plant through gene transient expression, comprising the following steps: transiently expressing a sequence-specific nuclease specific to the target fragment in the cell or tissue of the plant of interest, wherein the sequence-specific nuclease is specific to the target site and the target site is cleaved by the nuclease, thereby the site-specific modification of the target site is achieved through DNA repairing of the plant.