A physiological detection structure includes a main body, a slidable member, and a detection module. The slidable member is combined to a surface of the main body. The main body includes at least one opening formed therein. The slidable member has an extra preserved length. The detection module is combined to the slidable member and exposed outside the opening of the main body. As such, the detection module is allowed to displace by means of the extra preserved length of the slidable member to provide the detection module with an effect of changing the site where detection is to be made for detecting physiological signals of different sites of the human body. Further, with such an operatively-disconnected arrangement, the stability of the detection module is ensured and discomfort of human body caused by the detection module being long retained on and depressing a fixed location of the skin is eliminated.