CHEN, YI LIN,陈怡伶,陳怡伶,TSAY, HSIN SHENG,蔡新声,蔡新聲,WAY, TZONG DER,魏宗德,魏宗德,LEE, PO LEI,李柏磊,李柏磊,YU, ZHUO YUAN,游卓远,游卓遠,CHEN, CHIEN HSU,陈建旭,陳建旭
申请号：
TW100135058
公开号：
TW201313242A
申请日:
2011.09.28
申请国别(地区):
TW
年份:
2013
代理人:
摘要：
The present invention was carried out to investigate the protective effect of Saussurea involucrate and Rutin against D-galactose (D-gal)-induced brain injury in mice. Protein expression was determined by Western blot. Apoptotic cells was determined by H&E stain. D-galactose aging animal treatment was used as a model system to study the anti-oxidant effects of Saussurea involucrate and Rutin in vivo. Pretreatment with Saussurea involucrate and Rutin increased cell viability and decreased membrane damage, generation of reactive oxygen species and degradation of poly (ADP-ribose) polymerase in D-galactose-treated mice and also decreased the expression of cyclooxygenase-2, via downregulation of NF-kappaB, resulting in a decrease in lipid peroxidation. These results suggest that Saussurea involucrate and Rutin are potential antioxidant agents. In another set of experiments, we evaluated the effect of Saussurea involucrate and Rutin in a murine aging model, in which chronic systemic exposure to D-galactose (D-gal) causes the acceleration of senescence. Administration of Saussurea involucrate and Rutin (30 mg/kg/d and 10 mg/kg/d) for 6 weeks concomitant with D-gal injection significantly increased superoxide dismutase and glutathione peroxidase activities and decreased the MDA level in plasma and brain. Furthermore, we used H&E staining to quantify cell death within hippocampus: counting of pyknotic nuclei in H&E section. The result showed that percentage of pyknotic nuclei in the D-gal-treated mice were much higher than in control. These results suggest that Saussurea involucrate and Rutin exerts potent antiaging effects against D-gal in mice via antioxidative mechanisms.本發明揭示一種用於抗疲勞及抗老化之天山雪蓮萃取物、醫藥組成物及其用途。本發明實施例中,揭露一種有關含有低濃度,較佳為30mg/kg之天山雪蓮(Saussurea involucrate)乙酸乙酯層萃取物及30mg/kg其純化物芸香甘(Rutin)可經由抑制脂質過氧化產物丙二醛(MDA)的表現,並活化穀胱甘肽過氧化酶(GPx)的活性。同時,天山雪蓮乙酸乙酯層萃取物及Rutin亦可透過抑制NF-κB的表現量來降低第二型環氧酶(COX-2)之蛋白質表現量,並且降低細胞凋亡蛋白PARP及caspase-3的表現,進而達到保護神經細胞之作用。再者,在本發明實施例中亦揭露在老化鼠模式中進行含有天山雪蓮的灌食投與。另
