A method and a tray for production of a bio-membrane are disclosed in the present invention. The bio-membrane produced in the method and in the tray of the present invention can be processed into a mask, wherein processes for changing the shape of the bio-membrane are unnecessary. The mask produced from the bio-membrane can be efficient to maintain the skin, and to supply the moisture thereto.本發明係關於一種生物膜之培養容器及其製造方法,藉由該培養容器及該製造方法所生產之生物膜可後製成面膜,且不需經過更動形狀的製程,因此能提高良率;所製成之面膜能成為補充水份並保養皮膚之有效面膜。12...周壁121...凹階緣122...凸階緣123...槽孔124...凸栓13...突出物132...第一突出物133...第二突出物134...第三突出物232~234...開口20...生物膜