PROBLEM TO BE SOLVED: To provide a nisin-containing composition that is good in storage stability in a mildly acidic region and exerts an excellent antimicrobial effect without raising the cytotoxicity even when the concentration of the nisin is low.SOLUTION: This nisin-containing antimicrobial composition is characterized by including a (A) component of nisin and a (B) component of one or two or more kinds of cellulose derivatives selected from alkyl celluloses and hydroxyalkyl celluloses.