THE UNITED STATES OF AMERICA; AS REPRESENTED BY THE SECRETARY; DEPARTMENT OF HEALTH AND HUMAN SERVICES
发明人:
TRAN, ERIC,LU, YONG-CHEN,ROBBINS, PAUL F.,ROSENBERG, STEVEN A.
申请号：
CA2963362
公开号：
CA2963362A1
申请日:
2014.10.02
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
Disclosed are methods of isolating a TCR having antigenic specificity for a mutated amino acid sequence encoded by a cancer-specific mutation, the method comprising: identifying one or more genes in the nucleic acid of a cancer cell of a patient, each gene containing a cancer-specific mutation that encodes a mutated amino acid sequence； inducing autologous APCs of the patient to present the mutated amino acid sequence； co-culturing autologous T cells of the patient with the autologous APCs that present the mutated amino acid sequence； selecting the autologous T cells； and isolating a nucleotide sequence that encodes the TCR from the selected autologous T cells, wherein the TCR has antigenic specificity for the mutated amino acid sequence encoded by the cancer-specific mutation. Also disclosed are related methods of preparing a population of cells, populations of cells, TCRs, pharmaceutical compositions, and methods of treating or preventing cancer.
