At the same time the simplicity of the elastic tissue which keeps normal structure for example in the person who includes thing, and the elastic tissue which offer efficient playback technology transplantation possible artificial organization (, the artificial skin, the artificial blood vessel) the technology which makes production possible is offered. Concrete, DANCE and/or the cell which possesses elastic tissue regenerative capacity under the existing of fibulin-4 for example culture (, bloodless the fact that culture) it does clearly in the nutrient medium is included, production method of the elastic tissue DANCE and/or the artificial elastic tissue which includes fibulin-4 Elastic tissue regenerant DANCE and/or bloodless fibulin-4 is included it is clear the nutrient medium And the cell and the like which possesses the elastic tissue regenerative capacity which the DANCE manifestation vector, the fibulin-4 manifestation vector or the DANCE elicitor manifestation vector one side is introduced at least is offered.正常構造を保持する弾性線維の簡便且つ効率的な再生技術を提供すること、並びに弾性線維を含むヒトに移植可能な人工組織(例えば、人工皮膚、人工血管)の作製を可能とする技術を提供する。具体的には、DANCE及び/又はfibulin-4の存在下で弾性線維再生能を有する細胞を培養(例えば、無血清培地中で培養)することを含む、弾性線維の製造方法;DANCE及び/又はfibulin-4を含む人工弾性線維;弾性線維再生剤;DANCE及び/又はfibulin-4を含む無血清培地;並びにDANCE発現ベクター、fibulin-4発現ベクター又はDANCE誘導因子発現ベクターの少なくとも一方が導入された弾性線維再生能を有する細胞などを提供する。
