An immunogenic composition for use as a blood-stage malaria vaccine, a method of producing the immunogenic composition and a method of treatment of malaria are provided. The immunogenic composition includes isolated or purified merozoites, or red blood cells infected with merozoites, treated with centanamycin or tafuramycin A. The immunogenic composition does not include an adjuvant. A single dose of the immunogenic composition is sufficient to protect an animal against subsequent malaria infection by the same isolate, strain or species of Plasmodium used in the immunogenic composition, or by one or more heterologous isolates, strains or species of Plasmodium.La presente invención se refiere a un método in vitro para producir una composición inmunogénica, caracterizado porque incluye la etapa de tratar parásitos de paludismo en etapa sanguínea o glóbulos rojos infectados con las parásitos de paludismo en etapa sanguínea con centanamicina, tafuramicina A o un análogo o derivado de los mismo, para de esta manera producir la composición inmunogénica.
