The present invention relates to a biomaterial having enhanced rubber properties through natural cross-linkage of collagen and hyaluronic acid, a preparing method thereof, and a using method thereof. To this end, the present invention is characterized in that a rubber type biomaterial is prepared by natural cross-linkage of collagen and hyaluronic acid at a ratio of 2:1 to 7:1. The present invention as described above creates a novel material exhibiting rubber properties by providing the conditions for natural cross-linkage rather than chemical or physical cross-linkage and mixing at the optimum ratio of collagen and hyaluronic acid considering their molecular structural features, thereby significantly improving quality and reliability of products, thus meeting a variety of user or customer needs to present a good image.
