Perfusion device (1) forming a unitary closed circuit system, comprising a bag (2) formed by a flexible envelope defining an inner chamber and by perfusion means (3) in fluidic communication with the inner chamber through of a tube (4) connected to an outlet (5) of the bag outlet (2), said inner chamber being provided with compartmentalization means (6) that are positioned to form a first and second compartments (7, 8 ) which contain or are intended to contain respectively a first and a second solution, said compartments (7, 8) being in fluidic communication with the orifice (5) respectively according to a first and according to a second flow paths, the second flow path leading into the first compartment (7), said first compartment (7) consisting of an upper chamber (9) that is disposed above the second compartment (8), and a lower chamber (10) which is arranged, at least in part, at the height and / or below the second compartment (8), the reversible flow shutter (11) interposing in the second flow path, said shutter being suitable, depending on the difference of pressure between the upper part and the lower part of the second flow path, to prevent the flow of the second solution during the infusion of the contents of the upper chamber (9) and to allow the flow of the second solution during the perfusion of the lower chamber content (10).Dispositivo (1) de perfusión que forma un sistema unitario de circuito cerrado, que comprende una bolsa (2) formada por una envoltura flexible que define una cámara interior y por unos medios (3) de perfusión en comunicación fluídica con la cámara interior a través de un tubo (4) conectado a un orificio (5) de salida de la bolsa (2), dicha cámara interior estando provista de unos medios (6) de compartimentación que están colocados para formar un primer y un segundo compartimentos (7, 8) que contienen o están destinados a contener respectivamente una primera y una segunda soluciones, dichos compartimentos (7, 8) estando en comunicac
