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sequências peptídicas miméticas de taenia saginata como alvos antigênicos e imunogênicos
专利权人:
UNIVERSIDADE FEDERAL DO PARANÁ
发明人:
JANAÍNA CAPELLI PEIXOTO,JULIANA FERREIRA DE MOURA,LARISSA MAGALHÃES ALVARENGA,RAFAELA LENZI FOGAÇA,VANETE THOMAZ SOCCOL
申请号:
BR102014017747
公开号:
BR102014017747A2
申请日:
2014.07.18
申请国别(地区):
BR
年份:
2016
代理人:
摘要:
Selection, description and application of peptide sequences in the identification of animals infested by Platyhelminthes the cysticercosis is an important zoonosis caused by the development of metacestodeos Taenia saginata (Cysticercus bovis) in bovine tissues. This disease causes huge losses to the livestock cutting and is considered to be a major public health problem.Diagnostic tests sensitive and accurate for the detection of parasitism in the live animal and vaccination programmes remains a challenge.The objective of this study was to utilize peptides exposed on surfaces of bacteriophages, screened in ELISA immunoassays by assessing bovine sera positive and negative for cysticercosis in an attempt to find a good antigen to be used in ante mortem detection of Cysticercosis.Using the technology of phage display, we have 8 mimotopos which have high reactivity compared to the serum positive for bovine cysticercosis. Among the sequences presented by these clones, the amino acid had a higher prevalence were proline, threonine, alanine and arginine.When these amino acids were replaced by alanine or serine, in the test of ALA scan performed with the mimotopos TSAG 1, 2 and 3 TSAG TSAG occurred decrease of reactivity to these peptides, highlighting the importance of these amino acids.Availando antigenic potential of mimotopos obtained front the bovine sera positive and negative, we have obtained a mimotopo, TSAG 3 with high correlation in relation to the crude antigen of C. bovis in the identification of animals both positive and negative.Our results demonstrate that phage display technology is efficient in the selection and identification of peptides reactive to antibodies of interest, as well as the identification of cattle infected by C. bovis.seleção, descrição e aplicação de sequências peptídicas na identificação de animais parasitados por platyhelminthes a cisticercose é uma importante zoonose provocada pelo desenvolvimento dos metacestodeos de taenia saginata (cy
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中国工程科技知识中心
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