The present invention relates to a method for activating NKT cells and thus proliferating the NKT cells, producing IFN-[gamma] and/or producing NKT cell ligand-pulsed human CD14-positive cells capable of strongly inducing cytotoxic activity. More specifically, this method is characterized by comprising culturing isolated CD14-positive cells in a medium that contains an NKT cell ligand and GM-CSF but substantially does not contain IL-4. Further, the present invention relates to a method for producing an NKT cell ligand-pulsed human CD14-positive cell line. More specifically, the method is characterized by comprising culturing isolated CD14-positive cells in a medium that contains an NKT cell ligand but substantially does not contain GM-CSF and IL-4. Furthermore, the present invention relates to a cell preparation comprising NKT cell ligand-pulsed human CD14-positive cells or an NKT cell ligand-pulsed human CD14-positive cell line and a pharmaceutical use of said cell preparation.本發明係關於活化NKT細胞使NKT細胞增殖、IFN-γ產生及/或強力誘導細胞毒活性之NKT細胞配位體脈衝人類(Ligand pulsed human)CD14陽性細胞之製造方法，具體為該方法的特徵為含有將經分離的CD14陽性細胞在含有NKT細胞配位體及GM-CSF，而實質上未含有IL-4的培養基中進行培養。又，本發明係關於製造NKT細胞配位體脈衝人類CD14陽性細胞株的方法，具體為該方法的特徵為含有將經分離的CD14陽性細胞在含有NKT細胞配位體，而實質上未含有GM-CSF及IL-4的培養基中進行培養。本發明係又關於含有NKT細胞配位體脈衝人類CD14陽性細胞或NKT細胞配位體脈衝人類CD14陽性細胞株之細胞製劑或其醫藥用途。
