An object of the present invention is to provide a method for microbiologically producing zeaxanthin of high concentration at low cost while suppressing production of gluconic acid. Specifically, the present invention relates to a method for producing carotenoids containing zeaxanthin, the method comprising a step of culturing a bacterium producing carotenoids containing zeaxanthin in a medium containing biotin, wherein a concentration of produced gluconic acid and a concentration of produced zeaxanthin in a culture liquid after the end of culture are lower and higher, respectively, than those in a culture liquid after the end of culture in a biotin-free medium.
