A combination of a puncture device (101) and a puncture needle cartridge (102), the puncture device (101) comprising a housing (602) having an opening at its front end to receive said cartridge (102) of puncture needle slidably in the housing, said puncture needle cartridge (102) being constituted by a lancet (104) comprising a lancet body (203) having a puncture needle (301) and a protective cap (202) puncture needle to protect the puncture needle (301), and a puncture needle holder (201) that has an opening (401) and holds the lancet body (203) so that the lancet body can move in the support (201), in which said lancet body (203) also includes an arm means (302) that is shaped so that one end (305) thereof is connected to the lancet body (203) while the other end (306) is open so that it widens towards the extr front emo of the lancet body (203), and be elastically deformable outward or towards the center of the lancet body (203); said puncture needle holder (201) is a cylindrical support having a hookable means (403) that cooperates with the arm means (302), and holds the lancet body (203) in a movable manner along its direction axial, from an initial position of the lancet body (203) where the arm means is positioned behind the engaging means (403), in particular from a first position as a puncture preparation position, to a second position where the needle ( 301) puncture protrudes; the puncture needle protection cover (202) is arranged to prevent movement of the lancet body (203) with respect to the puncture needle cartridge (102) once the puncture needle cartridge (102) is inserted into the casing (602) of the puncture device (101) and to allow movement of the lancet body (203) with respect to the puncture needle cartridge (102) when the puncture needle protection cover (202) is removed from the puncture needle cartridge (102), said lancet body (203) is constituted so that, when moved from the first position along the puncture direction, said arm means (302)