FORMULATION (FORMULE OU PRESCRIPTION D'INGREDIENTS) D'UN PRODUIT ANTI-AGE CONCU POUR L'ANTI-AGE/L'INVERSION D'AGE ET LA CORRECTION DES MALADIES ASSOCIEES AU VIEILLISSEMENT PAR LE BIAIS DE LA REACTIVATION DE L'ENZYME DE TELOMERE
Object. To study the influence of Astragaloside on the activity of telomeraseof senile human embryolung fibroblasts (HELF) and the expression of anti-aging gene klotho mRNAMethod in vitroculture HELF, Astragaloside was added into experimental groups from the 50 'sgeneration of cells,inverted microscope observes cell morphology, MTT method checks the cellvitality, Dimiri detectsGalactose glucoside enzyme activity, TRAP-PCR Silver staining detectstelomerase activity, RT-PCRdetects klotho mRNA expression Results Comparing with youth group both cellvitality and klothomRNA expression in senile group decreased (P<；0 01), .beta.-Galactose glucosideenzyme activityincreased (P<；0 01), Comparing with senile group Astragaloside could reduce.beta.-Galactose glucosideenzyme activity (P<；0.01), improved cell vitality, telomerase activity andklotho mRNA expression(P<；0.01) Conclusion Astragaloside plays the role of anti-aging in HELF cellsby chancing celltelomerase activity and klotho gene expression"Key words" Astragaloside； telomerase； klotho, senile human embryo lungfibroblastsThe study on Anti-aging effect of Astragalus is more common 1 ) in vivo study2) serumPharmacology study. This experiment uses replicative senile human embryo lungfibroblast cells(human embryonic lung fibroblast, HELF) as the object of study, makes themonomer composition ofastragalus¨Astragaloside act directly on senile cells and observes itsinfluence on the cell senescenceand its mechanism of anti-aging effect, this provides reliable theoretical andexperimental basis forconfirming the anti-aging effect of Astragaloside i~ and its MolecularPharmacology research.1 Materials and Methods1 1 Experimental Groups and ProcessingHELF cells routinely are divided as one to two and cultured when generationscontinue The cellsyounger than 35th generation represent as young cells, older than 50thgeneration represent as senilece
