Young Chul Park;Amy McDonald;Camille Pierre Dubois;Sonia Lall;Sung-Yong Harrison Yoon
发明人:
Sung-Yong Harrison Yoon,Young Chul Park,Amy McDonald,Camille Pierre Dubois,Sonia Lall
申请号：
US13251960
公开号：
US09167840B2
申请日:
2011.10.03
申请国别(地区):
US
年份:
2015
代理人:
摘要：
A method of preparing cocoa oligomeric procyanidins from cocoa cell cultures grown in the presence of monosaccharide can increase production of procyanidins as follows: culturing cells sufficient to result in production of cocoa oligomeric procyanidins at a first rate； and introducing a monosaccharide to the cells sufficient for inducing the cells to produce the cocoa oligomeric procyanidins at a second rate that his higher than the first rate. The method can further include extracting the cocoa oligomeric procyanidins from the cells, and such extracting can occur between 1 day to 21 days after introduction of the monosaccharide. Optionally, the monosaccharide can be introduced in an amount about 0.5% to about to about 20% by volume of the culture medium. The monosaccharide can be glucose, sucrose, fructose, or the like. The monosaccharide can be introduced during or after a last phase of an exponential growth state.
