<;P>;PROBLEM TO BE SOLVED: To provide a method for cleaning breeding water, which does not utilize a biodegradable plastic and safely, stably and continuously removes nitrate nitrogen by a simple operation. <;P>;SOLUTION: The method for cleaning breeding water includes a deoxygenation process for reducing the amount of oxygen dissolved in a certain amount of water contained in breeding water W<;SB>;2<;/SB>;collected from a breeding water tank T, a carbon dioxide dissolution process for dissolving carbon dioxide in breeding water W<;SB>;2<;/SB>;, a nitrogen fixation process for passing the breeding water W<;SB>;2<;/SB>;having passed through the deoxygenation process and the carbon dioxide dissolution process to a nitrogen fixation tank 3A composed of a filter medium assembly and packed with a microorganism carrier 32 and a breeding water supply process for supplying returning breeding water W<;SB>;3<;/SB>;having passed through the nitrogen fixation process to the breeding water tank T. <;P>;COPYRIGHT: (C)2010,JPO&INPIT