A multi-modality imaging device is provided for use in surgical procedures. The disclosed imaging device incorporates a line of sight surface tissue imaging device and a sub-surface tissue imaging device into a single instrument to facilitate sub-surface surgical procedures. The line of sight surface tissue imaging device may be movably mounted relative to the sub-surface tissue imaging device and movable between a retained position and a deployed position offset from a longitudinal axis of the sub-surface tissue imaging device. Alternatively, the line of sight surface tissue imaging device may be stationary and concentric around the sub-surface tissue imaging device. There is also disclosed a system including a multi-modality imaging device mounted on a surgical instrument.