PENCHEL, RICARDO MIGUEL,PENCHEL, Ricardo Miguel,REIS, JOCEMAR PALAURO DOS,REIS, Jocemar Palauro dos,OLIVEIRA, MILA LIPARIZE DE,OLIVEIRA, Mila Liparize de
申请号：
BRBR2013/000483
公开号：
WO2015/066779A1
申请日:
2013.11.11
申请国别(地区):
WO
年份:
2015
代理人:
摘要：
The present utility model aims at providing a bioreactor for in vitro, temporary immersion plant culture, comprising: (i) an upper container (2) having transparent walls for the propagation material, preferably eucalyptus explants, the container (2) being provided with (a) diffusers for gaseous exchange with the outer environment, (b) humidifier (12), and (c) artificial illumination (14) (ii) a lower container (3) for the liquid nutrient medium, having transparent walls, an aluminium tray (15) and a water and/or nutrient medium inlet (13) located in the bottom of said container (3) (iii) air/oxygen/carbon dioxide injection/withdrawal points (4) located in the transparent cover (5) (iv) additional carbon dioxide injection point (11) with a dripping tube, near the bottom of said upper container (2) (v) hermetic connection means (6) between the upper and lower containers, allowing the nutrient medium to be supplied to and drained from the upper container (vi) a support for the screen (7) carrying the propagation material, with the option of using screens having different mesh sizes (vii) means (8) for locking the cover in order to seal the upper container hermetically (viii) means (9) for locking the upper container so that it is hermetically closed on the lower container and (ix) means (10) for pneumatically conveying the liquid nutrient medium between the upper container and the lower container.Le présent modèle dutilité vise à obtenir un bioréacteur pour la culture in vitro de plantes, du type à immersion temporaire, comprenant: (i) un récipient supérieur (2) à parois transparentes pour la matière en propagation, de préférence des explants deucalyptus, ledit récipient (2) comportant (a) des diffuseurs déchange gazeux avec lenvironnement extérieur, (b) un humidificateur (12), et (c) un éclairage artificiel (14) (ii) un récipient inférieur (3) à parois transparentes pour le milieu nutritif liquide, présentant un plateau daluminium (15) et un point (13) dentrée deau et
