The invention discloses a kind of polyethyleneglycol modified interferon conjugate, preparation method, and the pharmaceutical composition of the interferon conjugate containing therapeutically effective amount, and its purposes in preparation antiviral and antitumor, immunoregulation medicament.本發明公開了一種聚乙二醇修飾的干擾素偶聯物,其製備方法,以及含有治療有效量的該干擾素偶聯物的藥物組合物,及其在製備抗病毒、抗腫瘤、免疫調節藥物中的用途。