The present invention provides a production method of a pluripotent spheroid cell, comprising introducing a reprogramming peptide containing a nuclear localization signal into a differentiated cell； a preparation for induction of a pluripotent spheroid cell from a differentiated cell, which contains the reprogramming peptide, and the like. According to the present invention, a somatic cell can be reprogrammed without using reprogramming factors including transcription factors such as Oct3/4, Sox2 and the like, and low-molecular-weight compounds that promote reprogramming such as HDAC inhibitors and the like, and an induced pluripotent stem cell-like cell, a reprogrammed cell or a transdifferentiated cell can be obtained from a somatic cell.
