Федеральное государственное бюджетное учреждение "Российский кардиологический научно-производственный комплекс" Министерства здравоохранения Российской Федерации (RU)
发明人:
Дергилев Константин Владимирович (RU),Рубина Ксения Андреевна (RU),Сысоева Вероника Юрьевна (RU),Акчурин Ренат Сулейманович (RU),Ткачук Всеволод Арсеньевич (RU),Парфенова Елена Викторовна (RU)
申请号：
RU2013139034/10
公开号：
RU2013139034A
申请日:
2013.08.22
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. A method for obtaining progenitor cells from mammalian myocardial samples comprising grinding samples landing them on culture dishes coated, ensuring their adhesion and culturing milled samples in conditions of hypoxia when oxygen concentrations of 0.1-15% within 6-36 hours form a cell culture, extracting cells from the cell culture by means of its enzymatic treatment, culturing cells recovered on plates coated, ensuring the formation of cell aggregates, followed fermentate ivnoy processing cell aggregates for myocardial progenitor cells, wherein as a coating cup, ensuring the adhesion of crushed samples using a collagen and / or fibronectin and / or laminin, and / or gelatine and / or fibrin in an amount of 0.1- 1000 mcg / cm, and as the cover plates, ensuring the formation of cell aggregates, using poly-D (L) -lysine and / or poly-ornithine in an amount of 0.1-1000 mg / see 2. The method of claim. 1, characterized in that the grinding is carried myocardial samples to obtain pieces mm.3 1-10. A method according to claim 1, characterized in that the cultivation is carried out in myocardial sample medium comprising:. 0,1-99,9% IMDM, and / or DMEM, and / or DMEM-F12, and / or MEM, and / or Myelocult, and / or RPMI, and 0.01-50% fetal calf serum and / or horse, the cultivation process is performed in the shift 0.1-100% medium at least once every 10 dney.4. A method according to claim. 3, characterized in that the medium additionally comprises 0.01-3% penicillin-streptomycin and / or 0.01-1 mM 2-mercaptoethanol, and / or 0.01-5% L-glutamina.5. The method of claim. 1, characterized in that culturing cells extracted prior to forming1. Способ получения прогениторных клеток из образцов миокарда млекопитающего, включающий измельчение образцов, посадку их на культуральные чашки с покрытием, обеспечивающим их адгезию, и культивирование измельченных образцов в условиях гипоксии при концентрации кислорода 0,1-15% в течение 6-36 ч с образованием клеточной культуры, извлечение
