Phytase, methods for producing a phytase variant and a fermentation product, for enhancing the nutritional value of an animal feed, and for the treatment of plant proteins, isolated nucleic acid sequence, nucleic acid construct, recombinant expression vector, cell recombinant host, transgenic microorganism, or products, or elements thereof, composition, process for reducing phytate levels in animal waste, and use of phytase.
SOEREN FLENSTED LASSEN,LARS KOBBEROEE SKOV,LEONARDO DE MARIA,ESBEN PETER FRIIS,TOMOKO MATSUI,ALLAN NOERGAARD,JESPER VIND
申请号：
BRPI0919314
公开号：
BRPI0919314A2
申请日:
2009.09.28
申请国别(地区):
BR
年份:
2015
代理人:
摘要：
The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensitiliby, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile； and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed(such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
