Duncan J. Stewart,David Courtman,Seth Mount,Darryl Davis
申请号：
US15608691
公开号：
US20170340678A1
申请日:
2017.05.30
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Methods for generating serum-free and/or xenogen-free cardiac explant-derived stem cells (EDC) are provided. These methods may include providing an initial cardiac explant, which has been minced and digested plating the initial cardiac explant culturing the plated cardic explant in serum-free and xenogen-free medium harvesting EDC cells surrounding or emerging from the plated cardiac explant and optionally performing static expansion of harvested EDC cells in serum-free and xenogen-free media. Serum-free and/or xenogen-free cardiac EDC cells produced by these methods, as well as methods and uses thereof for the treatment of heart failure in a subject in need thereof, are also provided.
