Provided are a method, a computer program and a device for evaluating a lacrimal fluid layer non-invasively and objectively while reducing a load more than when measuring BUT and being adapted to a new standard for the diagnosis of dry eye.The method, computer program, and device include a step of detecting a time when color information in a predetermined region in a lacrimal fluid layer interference fringe image changes by a threshold value or more as an eyelid opening time, a step of extracting an image at the time when the predetermined time has passed from the eyelid opening time as an extracted image from the lacrimal fluid interference fringe images, a determination value calculation step of calculating a determination value from the color information in a pixel in a local region by scanning a predetermined target region in the extracted image, a step of detecting a corresponding pixel by comparing the calculated determination value and a threshold value, and an evaluation step of evaluating lacrimal fluid layer breakdown by comparing the detected pixel and a threshold value.
