A method, a computer program and a device for evaluating a tear fluid layer non-invasively. The method, computer program, and device include a step of detecting a time when color information in a predetermined region in a tear fluid layer interference fringe image changes by a threshold value or more as an eyelid opening time, a step of extracting an image at the time when the predetermined time has passed from the eyelid opening time as an extracted image from the tear fluid interference fringe images, a determination value calculation step of calculating a determination value from the color information in a pixel in a local region by scanning a predetermined target region, a step of detecting a corresponding pixel by comparing the calculated determination value and a threshold value, and an evaluation step of evaluating tear fluid layer breakdown by comparing the detected pixel and a threshold value.