Synthetic gene for expressing Sm-14 in Pichia pastoris, methods for producing and purifying Sm-14 and the use thereof as a vaccine and diagnostic medium
Andrew J. G. Simpson;Celso Raul Romero Ramos;Miriam Tendler
发明人:
Miriam Tendler,Celso Raul Romero Ramos,Andrew J. G. Simpson
申请号：
US13824378
公开号：
US09193772B2
申请日:
2011.09.13
申请国别(地区):
US
年份:
2015
代理人:
摘要：
The production of recombinant proteins using a synthetic gene for increased expression of the protein in Pichia pastoris is disclosed. More specifically, disclosed is a process for producing a recombinant Sm14 protein of Schistosoma mansoni, by providing a synthetic gene for increased production of Sm14 protein, producing the gene and genetically manipulating the Pichia pastoris strain with the gene in order to produce a vaccine effectively. Improved methods are also provided for producing and purifying this protein from P. pastoris cells, which can be scaled for industrial production.
