TENDLER, MIRIAM,SIMPSON, ANDREW J. G.,RAMOS, CELSO RAUL ROMERO
申请号：
CA3050981
公开号：
CA3050981A1
申请日:
2018.01.22
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
The present invention pertains to the field of the production of recombinant proteins, using a synthetic gene for increased expression of the protein in Pichia pastoris. More specifically, the invention describes the production of the recombinant Sm14 protein of Schistosoma mansoni, with the creation of a synthetic gene for increased expression of this protein, which gene was cloned under the control of two types of Pichia pastoris promoters: methanol-induced promoter (AOX1) and constitutive promoter (GAP). Strains of Pichia pastoris were genetically engineered with these constructs to produce the Sm14 vaccine antigen efficiently. Improved methods are also provided for producing and purifying this protein from P. pastoris cells, which can be scaled for industrial production.
