Williams Loren D.,Fang Po-Yu,Hsiao Chiaolong,Williams Justin
申请号：
US201414911645
公开号：
US9932563(B2)
申请日:
2014.09.11
申请国别(地区):
美国
年份:
2018
代理人:
Smith, Gambrell & Russell, LLP
摘要：
A combined packing and assembly method that efficiently packs ribonucleic acid (RNA) into virus like particles (VLPs) has been developed. The VLPs can spontaneously assemble and load RNA in vivo, efficiently packaging specifically designed RNAs at high densities and with high purity. In some embodiments the RNA is capable of interference activity, or is a precursor of a RNA capable of causing interference activity. Compositions and methods for the efficient expression, production and purification of VLP-RNAs are provided. VLP-RNAs can be used for the storage of RNA for long periods, and provide the ability to deliver RNA in stable form that is readily taken up by cells.
