Provided is a vector comprising first and second recombinase recognition sequences, wherein the recombinase recognition sequences, and any sequence between the recombinase recognition sequences, are derived from plant species, wherein the vector is capable of producing a minicircle DNA molecule in the presence of a suitable recombinase, and wherein the vector further comprises, between the recombinase recognition sequences, at least one T-DNA borderlike sequences. The recombinase recognition sequence can be LoxP-like sequences derived from a plant species. Further provided are methods of using the vectors to produce transgenic plants.
