A decontamination operation chamber is partitioned into a first operation chamber and a second operation chamber. A connecting portion that connects the first operation chamber and the second operation chamber can be closed. The pressure of the first operation chamber and the second operation chamber is adjusted by a pressure-adjusting mechanism. When the connecting portion is opened and the first operation chamber and the second operation chamber are connected, an inlet portion for the first operation chamber and an outlet portion for the second operation chamber are closed and air flow is generated from the second operation chamber to the first operation chamber by the pressure-adjusting mechanism. When carrying an object into an aseptic manipulation chamber, the object is housed in the first operation chamber and a first decontamination operation is performed, then the object is transferred to the second operation chamber and a second decontamination operation is performed.
