PROBLEM TO BE SOLVED: To provide a method for simply and rapidly obtaining yeast producing predetermined free fatty acid or fatty acid ester from selection target yeast groups and a method for producing liquors and food by using the yeast.SOLUTION: The method includes adding a color developer for free fatty acids using acyl activating enzyme to each medium cultivating divided selection target yeast groups, evaluating the magnitude of the concentration of the free fatty acids from the degree of intensity of the color development generated by the addition of the agent and obtaining a desired free fatty acid-producing yeast from each medium based on the magnitude of the concentrations of the free fatty acids.【課題】選抜対象酵母群から所定の遊離脂肪酸若しくは脂肪酸エステルを生成する酵母を簡便且つ迅速に取得する方法及びこの酵母を用いた酒類若しくは食品の製造方法を提供する。【解決手段】選抜対象酵母群を区分して夫々培養している各培地にアシル活性化酵素を用いた遊離脂肪酸発色剤を添加し、この添加により生ずる発色の濃淡度合から各培地に含まれる遊離脂肪酸の濃度の大小を求めてこれらの各培地の遊離脂肪酸の濃度の大小を求め、この遊離脂肪酸の濃度の大小を基にこれらの各培地から所望の遊離脂肪酸を生成する酵母を得る。【選択図】図2